Construction of nanofibrous scaffolds with interconnected perfusable microchannel networks for engineering of vascularized bone tissue

• DOI: 10.1016/j.bioactmat.2021.02.033
• Publication Date: 2021-03-14T07:30:18Z
• Authors: Jiani Gu; Qianqian Zhang; Mengru Geng; Weizhong Wang; Jin Yang; Atta ur Rehman Khan; Haibo Du; Zhou Sha; Xiaojun Zhou; Chuanglong He
• Keywords: Microchannel; Microangiography

Vascularization and bone regeneration are two closely related processes during reconstruction. A three-dimensional (3D) scaffold with porous architecture provides a suitable microenvironment for vascular growth formation. Here, we present simple general strategy to construct nanofibrous poly(l-lactide)/poly(ε-caprolactone) (PLLA/PCL) interconnected perfusable microchannel networks (IPMs) based on 3D printing technology by combining the phase separation sacrificial template methods. The regular customizable patterns within scaffolds (spacings: 0.4 mm, 0.5 0.6 mm; diameters: 0.8 1 1.2 mm) were made investigate effect of structure angiogenesis osteogenesis. results subcutaneous embedding experiment showed that 0.5/0.8-IPMs (spacing/diameter = 0.5/0.8) 0.5/1-IPMs 0.5/1) exhibited more network formation as compared other counterparts. After loading endothelial factor (VEGF), [email protected]/0.8 prompted better human umbilical vein cells (HUVECs) migration neo-blood vessel formation, determined Transwell migration, scratch wound healing, chorioallantoic membrane (CAM) assays. Furthermore, microangiography rat cranial defects experiments demonstrated performance in new protected]/1 scaffold. In summary, our suggested could be tailored an adjustable caramel-based strategy, combination perfusion angiogenic factors significantly enhance vascularization regeneration.