Marginal sealing around integral bilayer scaffolds for repairing osteochondral defects based on photocurable silk hydrogels

• DOI: 10.1016/j.bioactmat.2021.04.005
• Publication Date: 2021-04-19T14:11:55Z
• Authors: Xiaolin Wu; Mingliang Zhou; Fei Jiang; Shi Yin; Sihan Lin; Guangzheng Yang; Yuezhi Lu; Wenjie Zhang; Xinquan Jiang
• Keywords: Fibroin; Articular cartilage repair

Osteochondral repair remains a major challenge in current clinical practice despite significant advances tissue engineering. In particular, the lateral integration of neocartilage into surrounding native cartilage is difficult and inadequately addressed problem that determines success repair. Here, novel design an integral bilayer scaffold combined with photocurable silk sealant for osteochondral reported. First, we fabricated layer resembling surface morphology mechanical strength BMP-2-loaded porous subchondral bone facilitated osteogenic differentiation BMSCs. Second, TGF-β3-loaded methacrylated fibroin (Sil-MA) exhibiting biocompatibility good adhesive properties was developed confirmed to promote chondrocyte migration differentiation. Importantly, this Sil-MA hydrogel provided bridge between then guided new grow towards replace degraded from early stage knee Thus, regeneration superior were achieved vivo by using composite. These results demonstrate approach marginal sealing around scaffolds has tremendous potential use regeneration.