An engineered lamellar bone mimicking full-scale hierarchical architecture for bone regeneration

• DOI: 10.1016/j.bioactmat.2023.03.024
• Publication Date: 2023-04-08T04:07:34Z
• Authors: Tao Yang; Zhichao Hao; Zhenzhen Wu; Binxin Xu; Jiangchen Liu; Le Fan; Qinmei Wang; Yanshan Li; Dongying Li; Sangzhu Tang; Chuanzi Liu; Weichang Li; Wei Teng
• Keywords: Bone matrix; Bone Formation

Lamellar bone, compactly and ingeniously organized in the hierarchical pattern with 6 ordered scales, is structural motif of mature bone. Each scale exerts an essential role determining physiological behavior osteogenic bioactivity Engineering lamellar bone full-scale hierarchy remains a longstanding challenge. Herein, using bioskiving mineralization, we attempt to engineer compact constructs resembling Through systematically investigating effect mineralization on physicochemical properties bioactivities multi-sheeted collagen matrix fabricated by bioskiving, mimicry hierarchy-property relationship are elucidated. With prolongation performed bidirectional manner, i.e. first rising then descending, which supposed be related transformation mechanism from nonclassical classical crystallization. Construct mineralized 9 days can accurately mimic each efficiently promote osteogenesis. Bioinformatic analysis further reveals that this construct potently activates integrin α5-PI3K/AKT signaling pathway through mechanical biophysical cues, thereby repairing critical-sized defect. The present study provides bioinspired strategy for completely complex tissue, offers critical research model in-depth studying structure-function