Cartilage-inspired self-assembly glycopeptide hydrogels for cartilage regeneration via ROS scavenging

• DOI: 10.1016/j.bioactmat.2023.10.013
• Publication Date: 2023-10-18T22:53:58Z
• Authors: Zhijian Zhao; Xiaowei Xia; Junlin Liu; Mingzhuang Hou; Yang Liu; Zhangzhe Zhou; Yong Xu; Fan He; Huilin Yang; Yijian Zhang; Changshun Ruan; Xuesong Zhu
• Keywords: Scavenging

Cartilage injury represents a frequent dilemma in clinical practice owing to its inherently limited self-renewal capacity. Biomimetic strategy-based engineered biomaterial, capable of coordinated regulation for cellular and microenvironmental crosstalk, provides an adequate avenue boost cartilage regeneration. The level oxidative stress microenvironments is verified be vital tissue regeneration, yet it often overlooked biomaterials Herein, inspired by natural architecture, fibril-network glycopeptide hydrogel (Nap-FFGRGD@FU), composed marine-derived polysaccharide fucoidan (FU) naphthalenephenylalanine-phenylalanine-glycine-arginine-glycine-aspartic peptide (Nap-FFGRGD), was presented through simple supramolecular self-assembly approach. Nap-FFGRGD@FU hydrogels exhibit native cartilage-like characterized interwoven collagen fibers attached proteoglycans. Beyond structural simulation, fucoidan-exerted robust biological effects Arg-Gly-Asp (RGD) sequence-provided cell attachment sites realized functional reinforcement, synergistically promoted extracellular matrix (ECM) production reactive oxygen species (ROS) elimination, thus contributing chondrocytes-ECM harmony. In vitro co-culture with not only facilitated ECM anabolic metabolism but also scavenged ROS accumulation chondrocytes. Mechanistically, the chondro-protective induced rely on activation endogenous antioxidant pathways associated nuclear factor erythroid 2-related 2 (NRF2). vivo implantation successfully improved de novo generation 1.65-fold, concomitant coordinately restructured subchondral bone structure. Collectively, our ingeniously crafted bionic simultaneously rewired chondrocytes' function augmenting rebuilt microenvironment via preserving redox equilibrium, holding great potential engineering.