Hypoxia endothelial cells-derived exosomes facilitate diabetic wound healing through improving endothelial cell function and promoting M2 macrophages polarization

• DOI: 10.1016/j.bioactmat.2023.10.020
• Publication Date: 2023-11-10T23:50:41Z
• Authors: Peng Cheng; Xudong Xie; Liangcong Hu; Wu Zhou; Bobin Mi; Yuan Xiong; Hang Xue; Kunyu Zhang; Yuxiao Zhang; Yiqiang Hu; Lang Chen; Kangkang Zha; Bin Lv; Ze Lin; Chuanlu Lin; Guandong Dai; Yixin Hu; Tengbo Yu; Hankun Hu; Guohui Liu; Yingze Zhang
• Keywords: KLF4; Hypoxia

It is imperative to develop and implement newer, more effective strategies address refractory diabetic wounds. As of now, there currently no optimal solution for these Hypoxic human umbilical vein endothelial cells (HUVECs)-derived exosomes have been postulated promote wound healing, however, its effect molecular mechanism need further study. In this study, we aimed investigate whether hypoxic enhance healing in diabetics. Based on our high-throughput sequencing, differentially expressed lncRNAs (including 64 upregulated 94 downregulated lncRNAs) were found compared normoxic exosomes. Interestingly, lncHAR1B was one the prominently exosomes, showing a notable correlation with healing. More specifically, transmitted surrounding cells, which resulted significant increase level, thereby relieving dysfunction promoting switch from M1 M2 macrophages under high glucose conditions. Mechanistically, directly interacted transcription factor basic helix-loop-helix family member e23 (BHLHE23), subsequently led binding KLF 4 (KLF4) promoted KLF4 expression.