The non-Newtonian behavior of detergents during concentration is increased by macromolecules, in trans, and results in their over-concentration
Detergents concentration
0301 basic medicine
570
Polymers
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Detergents
Membrane Proteins
Membrane protein concentration
Non-Newtonian effect
03 medical and health sciences
616
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Molecular Biology
DOI:
10.1016/j.biochi.2022.09.004
Publication Date:
2022-09-07T15:39:01Z
AUTHORS (6)
ABSTRACT
Concentration of pure membrane proteins in detergent solution results in detergent concentration, albeit in unknown amounts. This phenomenon is observed in every lab working on membrane proteins, but has seldom been investigated. In this study, we explored the behavior of detergents mixed with membrane proteins during the step of sample concentration using centrifugal devices. We show that detergent over-concentrate with the presence of polymers, typically membrane or soluble proteins but also polysaccharides. The over-concentration of detergents depends on centrifugal force applied to the device. With the use of a specific dye, we observed the formation of a mesh on the concentrator device. Importantly, reducing the centrifugal speed allows to reduce the concentration of detergents when mixed to macromolecules, as tested with 3 different membrane proteins. All together, these results highlight the non-Newtonian behavior of detergents and provides a solid framework to investigators to improve drastically biochemical and structural studies of membrane proteins.
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