Xiaokang Liuwei Dihuang decoction ameliorates the immune infertility of male rats induced by lipopolysaccharide through regulating the levels of sex hormones, reactive oxygen species, pro-apoptotic and immune factors

• DOI: 10.1016/j.biopha.2021.111514
• Publication Date: 2021-05-02T08:58:54Z
• Authors: Xiaoyong Sun; Bin Wu; Liguo Geng; Jinfeng Zhang; Guozheng Qin
• Keywords: Decoction

Male immune infertility is a kind of disease that damages family life and happiness. The development novel methods treating male great significance. This study aimed to investigate the therapeutic effects Chinese medicine Xiaokang Liuwei Dihuang decoction on rats explored involved mechanisms. Model were established by lipopolysaccharide (LPS) injection. Anti-sperm antibody (AsAb) was detected ELISA assay testicular cell apoptosis evaluated TUNEL staining verify successful model establishment screen suitable doses decoction. Thirty then divided into five groups (n = 6 per group): Control, LPS, (15.12 g/kg, 30.24 g/kg 45.36 g/kg). Results HE showed compared with LPS group, treatments gradually improved morphology seminiferous tubules elevated number spermatogenic cells as increased. sperm levels testosterone, luteinizing hormone (LH) follicle stimulating (FSH) in serum 15.12 much higher than those group. staining, western blot interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), AsAb, malondialdehyde (MDA) toll-like receptor 2 (TLR2) tissue significantly decreased three groups. Compared Bax expression down-regulated well. In conclusion, might ameliorate induced through regulating sex hormones, reactive oxygen species, pro-apoptotic factors.