Modified citrus pectin prevents isoproterenol-induced cardiac hypertrophy associated with p38 signalling and TLR4/JAK/STAT3 pathway

• DOI: 10.1016/j.biopha.2021.112178
• Publication Date: 2021-09-27T05:50:53Z
• Authors: Yuan Li; Wei-Wei Zhou; Jia-Huan Sun; Hong-Xia Yang; Geng-Rui Xu; Yue Zhang; Qiu-Hang Song; Chuang Zhang; Wei-Zhe Liu; Xing-Chao Liu; Ai-Ying Li
• Keywords: Atrial natriuretic peptide; Brain natriuretic peptide

Modified citrus pectin (MCP) is a specific inhibitor of galectin-3 (Gal-3) that regarded as new biomarker cardiac hypertrophy, but its effect unclear. The aim this study to investigate the role and mechanism MCP in isoproterenol (ISO)-induced hypertrophy. Rats were injected with ISO induce hypertrophy treated MCP. Cardiac function was detected by ECG echocardiography. Pathomorphological changes evaluated haematoxylin eosin (H&E) wheat germ agglutinin (WGA) staining. hypertrophy-related genes for atrial natriuretic peptide (ANP), brain (BNP), β-myosin heavy chain (β-MHC), associated signal molecules analysed qRT-PCR western blotting. results show prevented ameliorated dysfunction structural disorder. also decreased levels ANP, BNP, β-MHC inhibited expression Gal-3 Toll-like receptor 4 (TLR4). Additionally, blocked phosphorylation Janus kinase 2 (JAK2) transducer activator transcription 3 (STAT3), it promoted p38. Thus, ISO-induced activating p38 signalling inhibiting Gal-3/TLR4/JAK2/STAT3 pathway.