Anti-inflammatory effects of the Aralia elata and Cirsium japonicum in Raw264.7 cells and in vivo colitis model in mice and dogs

• DOI: 10.1016/j.biopha.2022.113186
• Publication Date: 2022-05-25T21:11:35Z
• Authors: Ryunhee Kim; Md Sadikul Islam; Yeo-Jin Yoo; Ha-Young Shin; Jeong Ho Lee; Jeong-Hwi Cho; Yang-Gyu Park; Jinyoung Choi; Hyun-Jin Tae; Byung-Yong Park
• Keywords: Anti-inflammatory

Ulcerative colitis (UC) is a severe inflammatory disease that has spread throughout the world. Cirsium japonicum (CJ) and Aralia elata (AE) are natural herbs with potent antioxidative antidiabetics anti-inflammatory effects. In this investigation, we studied defensive role of combination CJ AE against LPS-induced inflammation in RAW 264.7 cells, dextran sulfate sodium (DSS)-induced mice, acetic acid-induced dogs. MTT assay was performed to identify toxic effect extracts. NO, MDA level also measured by NO assay. To measure pro-inflammatory protein expression, western blot performed. induce colitis, 3% DSS used for mice 6% acid Histopathology colonoscopy were executed detect pretreatment reduced MDA, expression proteins cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) 264.7. Compared separate doses AE, combined dose significantly clinical symptoms induced dogs including weight loss, bloody stool, shortening colon, severity degree histological damage colon. Therefore, these results indicated protective DSS-mediated colonic