Experimental study of 131I-caerin 1.1 and 131I-c(RGD)2 for internal radiation therapy of esophageal cancer xenografts

• DOI: 10.1016/j.biopha.2023.114891
• Publication Date: 2023-05-18T11:26:23Z
• Authors: Tiantian He; Juan Du; Keke Zhu; Yixuan Zhou; Zewei Xiao; Wenjie Liu; Weiwei Ren; Xiongying Liu; Tongsheng Chen; Wenjuan Liu; Zhuanming Chen; Guoying Ni; Xiaosong Liu; Tianfang Wang; Jiangtao Quan; Peipei Zhang; Jianwei Yuan
• Keywords: Clonogenic assay; MTT assay

The aim of this study was to analyze and compare the therapeutic effects 131I-caerin 1.1 131I-c(RGD)2 on TE-1 esophageal cancer cell xenografts.(1) in vitro antitumor polypeptides caerin c(RGD)2 were verified by MTT clonogenic assays. prepared chloramine-T (Ch-T) direct labeling, their basic properties measured. binding elution 1.1, 131I-c(RGD)2, Na131I (control group) cells studied through (2) antiproliferative effect cytotoxicity Na131I, detected Cell Counting Kit-8 (CCK-8) assay. (3) A nude mouse (TE-1) xenograft model established efficacy internal radiation therapy for cancer.(1) Caerin inhibited proliferation a concentration-dependent manner, with an IC50 13.00 µg/mL. polypeptide had no evident inhibitory cells. Therefore, significantly different (P < 0.05). assay showed that clonal decreased as concentration increased. Compared control group (drug 0 µg/mL), lower CCK-8 cells, while proliferation. two at higher concentrations assays stably bound rate 15.8 % ± 1.09 24 h 6.95 0.22 after incubation elution. 0.06 0.02 0.23 0.11 In vivo experiment, 3 days last treatment, tumor sizes phosphate-buffered saline (PBS) group, 131I 68.29 2.67 mm3, 61.78 3.58 56.67 5.65 58.88 1.71 14.40 1.38 60.14 0.47 respectively. other treatment groups, smaller 0.001). After tumors isolated weighed. weights PBS 39.50 9.54 mg, 38.25 5.38 38.35 9.53 28.25 8.50 9.50 4.43 34.75 8.06 lighter than those groups 0.01).131I-caerin has tumor-targeting properties, is capable targeted can be retained cytotoxic killing effect, effect. better suppressed growth pure c(RGD)2.