Lactobacillus plantarum modulate gut microbiota and intestinal immunity in cyclophosphamide-treated mice model

• DOI: 10.1016/j.biopha.2023.115812
• Publication Date: 2023-11-16T10:27:37Z
• Authors: Zhibo Zeng; Zonghao Huang; Wen Yue; Shah Nawaz; Xinzhu Chen; Jing Liu
• Keywords: Lactobacillus plantarum; Cyclophosphamide; Gut microbiota; Immunology; Butyrate; Probiotics; FOS: Clinical medicine; Immunology; Butyrate; Gut microbiota; 3. Good health; Gastrointestinal Microbiome; Intestines; Mice; RNA, Ribosomal, 16S; Animals; Cytokines; Cyclophosphamide; Lactobacillus plantarum

Gut microbiota (GM) contributes to the production of immune-regulatory molecules and cytokines. However, our understanding regarding intricate relationship between Lactobacillus plantarum GM on regulation immune function remained limited. To investigate effect an immunosuppressed mouse model, we employed cyclophosphamide treatment conducted various analysis including H&E (hematoxylin-eosin staining), immunohistochemistry, 16S rRNA gene sequencing, RT-PCR. Our results demonstrated that administration had significant immunoenhancing effects in immune-suppressed mice, as evidenced by restoration functional expression specific markers spleen increase number goblet cells intestine (P < 0.05). Microbial taxonomic revealed alterations gut composition, characterized a decrease richness Firmicutes proportion Verrucomicrobia Actinobacteria following treatment. Furthermore, significantly suppressed mRNA inflammatory cytokines 0.05), which were subsequently restored after plantarum. These observations provide valuable insights into complex interplay probiotics, microbiota, system functioning.