Electrospun polyacrylonitrile nanofibrous membranes for chitosanase immobilization and its application in selective production of chitooligosaccharides

• DOI: 10.1016/j.biortech.2011.11.101
• Publication Date: 2011-12-08T16:30:03Z
• Authors: Sujata Sinha; Sanjay R. Dhakate; Pankaj Kumar; R.B. Mathur; Pushplata Tripathi; Subhash Chand
• Keywords: 0106 biological sciences; 0301 basic medicine; Chitosan; Glucosamine; Time Factors; Glycoside Hydrolases; Hydrolysis; Acrylic Resins; Nanofibers; Temperature; Oligosaccharides; Membranes, Artificial; Hydrogen-Ion Concentration; Enzymes, Immobilized; 01 natural sciences; 03 medical and health sciences; Enzyme Stability; Animals; Recycling; Chromatography, Thin Layer; Biotechnology

Polyacrylonitrile nanofibrous membranes (PANNFM) were prepared by electrospinning from 10 wt.% of PAN solution and its surface was modified by amidination reaction. A new chitosan degrading enzyme from Aspergillus sp. was covalently immobilized on PANNFM. Immobilization efficiency of 80% was achieved by activating PANNFM surface for 30 min followed by 2 h treatment with enzyme solution. The optimum temperature and pH for immobilized enzyme were 50°C and 5.8, respectively. The immobilized chitosanase retained >70% activity after ten repeated batch reaction and could be stored up to 60 days at 4°C with minor loss in activity. Chitosan hydrolysis using different substrates were studied using immobilized chitosanase in batch conditions. Continuous selective production of chitooligosaccharides (dimer to hexamer) by changing the temperature was achieved by PANNFM-chitosanase.