Structural features and antioxidant activity of xylooligosaccharides enzymatically produced from sugarcane bagasse

• DOI: 10.1016/j.biortech.2012.09.112
• Publication Date: 2012-10-06T20:01:20Z
• Authors: Jing Bian; Feng Peng; Xiao-Peng Peng; Pai Peng; Feng Xu; Run-Cang Sun
• Keywords: Xylobiose; Bagasse; Pichia stipitis; Enzymatic Hydrolysis

Xylooligosaccharides (XOS) were prepared from xylan-rich hemicelluloses isolated by potassium hydroxide from sugarcane bagasse by hydrolysis with crude xylanase secreted by Pichia stipitis. Hydrolysis for 12h produced XOS with a maximum yield of 31.8%, equivalent to 5.29 mg mL(-1) in the hydrolyzate. XOS with degrees of polymerization (DP) from 2 to 4 (xylobiose, xylotriose, and xylotetraose) were the major components in the hydrolysates, whereas the oligosaccharides with higher DP of 5-6 (xylopentaose and xylohexose) showed a constant low level. FT-IR and NMR ((1)H, (13)C, HSQC) demonstrated that XOS contained Araf and 4-O-Me-α-D-GlcpA residues. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that the XOS exhibited concentration-dependent antioxidant activity. The results obtained indicate that the XOS produced from sugarcane bagasse can be employed in food-related applications.