One-step synthesis of high-yield biodiesel from waste cooking oils by a novel and highly methanol-tolerant immobilized lipase

• DOI: 10.1016/j.biortech.2017.03.086
• Publication Date: 2017-03-18T17:48:09Z
• Authors: Xiumei Wang; Xiaoli Qin; Daoming Li; Bo Yang; Yonghua Wang
• Keywords: 0106 biological sciences; Esterification; Biofuels; Methanol; Spectroscopy, Fourier Transform Infrared; Plant Oils; Cooking; Lipase; Enzymes, Immobilized; 01 natural sciences

This study reported a novel immobilized MAS1 lipase from marine Streptomyces sp. strain W007 for synthesizing high-yield biodiesel from waste cooking oils (WCO) with one-step addition of methanol in a solvent-free system. Immobilized MAS1 lipase was selected for the transesterification reactions with one-step addition of methanol due to its much more higher biodiesel yield (89.50%) when compared with the other three commercial immobilized lipases (<10%). The highest biodiesel yield (95.45%) was acquired with one-step addition of methanol under the optimized conditions. Moreover, it was observed that immobilized MAS1 lipase retained approximately 70% of its initial activity after being used for four batch cycles. Finally, the obtained biodiesel was further characterized using FT-IR, 1H and 13C NMR spectroscopy. These findings indicated that immobilized MAS1 lipase is a promising catalyst for biodiesel production from WCO with one-step addition of methanol under high methanol concentration.