A comparative analysis of heavy metal bioaccumulation and functional gene annotation towards multiple metal resistant potential by Ochrobactrum intermedium BPS-20 and Ochrobactrum ciceri BPS-26

• DOI: 10.1016/j.biortech.2020.124330
• Publication Date: 2020-10-28T16:38:11Z
• Authors: Babita Sharma; Pratyoosh Shukla
• Keywords: Metals, Heavy; 0211 other engineering and technologies; Humans; Molecular Sequence Annotation; 02 engineering and technology; Ochrobactrum; Bioaccumulation

The present study describes the heavy metal bioaccumulation potential of Ochrobactrum intermedium BPS-20 and Ochrobactrum ciceri BPS-26. A total of 27 isolates were retrieved from the soils of industrial areas and these two were selected based on their maximum metal tolerance. They can resist up to 2400 mg/L and 2000 mg/L of Lead and 850 mg/L and 1200 mg/L of Nickel respectively. The atomic absorption spectroscopic analysis showed considerably good bioaccumulation by O. intermedium BPS-20 (85.34% and 74.87%) and O. ciceri BPS-26 (71.20% and 88.48%) for Lead and Nickel respectively. The growth rate studies also demonstrated no inhibitory effects of heavy metals in the medium. Further the SEM analysis showed the presence of extracellular polymeric substances around bacterial cells. Moreover, the functional gene annotation confirmed the presence of ATPase, ABC, and HoxN/HupN/NixA families of transporters. Thus, both the isolates provide a better solution for the removal of metal pollutants.