Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse

• DOI: 10.1016/j.biortech.2021.125565
• Publication Date: 2021-07-14T16:13:48Z
• Authors: Taís Mayumi Kuniy...; Carlos Miguel Nób...; Viviane Borges Vi...; Diogo Robl; Bernadette Dora G...; Svetoslav Dimitro...; Elisabetta Tomé; Paula Mary O'Connor; Attilio Converti; Welington Luiz Ar...; Lívia Paula Silva...; Alessandro de Mel...; Paul David Cotter; Sarita Cândida Ra...; Ricardo Pinheiro ...
• Keywords: Bagasse; Pediococcus acidilactici; Hemicellulose

Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg·L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production.