Expression and characterization of α-L-arabinofuranosidase derived from Aspergillus awamori and its enzymatic degradation of corn byproducts with xylanase

• DOI: 10.1016/j.biortech.2023.129278
• Publication Date: 2023-06-07T18:11:11Z
• Authors: Chunyue Li; Heng Cao; Wei Wu; Gang Meng; Chunguang Zhao; Yunhe Cao; Jianmin Yuan
• Keywords: Aspergillus awamori; Husk

In this study, α-L-arabinofuranosidase (AF) from Aspergillus awamori was heterologously expressed in Pichia pastoris X33, with a 1-fold increase AF activity after codon and vector optimization. remained stable at 60-65 °C displayed broad pH stability range of 2.5-8.0. It also demonstrated considerable resistance to pepsin trypsin. Furthermore, compared xylanase alone, exhibited marked synergistic effect the degradation expanded corn bran, distillers' dried grains solubles, reducing sugars by 3.6-fold, 1.4-fold, 6.5-fold, respectively, degree synergy increasing 4.61, 2.44, 5.4, while vitro dry matter digestibility values were 17.6%, 5.2%, 8.8%, respectively. After enzymatic saccharification, byproducts converted prebiotic xylo-oligosaccharides arabinoses, thereby demonstrating favorable properties biomass its byproducts.