Construction of a synthetic microbial community based on multiomics linkage technology and analysis of the mechanism of lignocellulose degradation

• DOI: 10.1016/j.biortech.2023.129799
• Publication Date: 2023-09-27T06:25:41Z
• Authors: Jinmeng Chen; Yafan Cai; Zhi Wang; Zhengzhong Xu; Jia Li; Xiaotian Ma; Wei Zhuang; Dong Liu; Shilei Wang; Andong Song; Jingliang Xu; Hanjie Ying

The efficient degradation of lignocellulose is a bottleneck for its integrated utilization. This research performed species analysis and made functional predictions in various ecosystems using multiomics coupling to construct a core synthetic microbial community with efficient lignocellulose degradation function. The synthetic microbial community was employed to degrade corn straw via solid-state fermentation. The degradation mechanisms were resolved using proteomics. The optimum culture conditions included 10% inoculum level (w/v), 4% nitrogen source ratio and a fermentation time of 23 d. Under these conditions, the degradation rates of cellulose, hemicellulose, and lignin were 34.91%, 45.94%, and 23.34%, respectively. Proteomic analysis revealed that lignin 1,4-β-xylanase, β-xylosidase and endo-1,4-β-xylanase were closely related to lignocellulose degradation. The metabolic pathways involved in lignocellulose degradation and the functional roles of eight strains were obtained. The synthesis of a microbial community via multiomics linkage technology can effectively decompose lignocellulose, which is useful for their further utilization.