From molasses to purified α-ketoglutarate with engineered Corynebacterium glutamicum

• DOI: 10.1016/j.biortech.2024.131803
• Publication Date: 2024-11-12T12:07:06Z
• Authors: Lars Halle; Daniela Höppner; Marvin Doser; Christian Brüsseler; Jochem Gätgens; Niclas Conen; Andreas Jupke; Jan Marienhagen; Stephan Noack
• Keywords: Corynebacterium glutamicum; Corynebacterium; Genetically engineered

α-ketoglutarate (AKG) is a valuable dicarboxylic acid with multiple applications in the food, pharmaceutical, and chemical industries. Its synthesis associated toxic by-products, low specificity, high energy input. To create more environmentally friendly sustainable alternative, microbial production process for AKG was developed. Four potential producer strains were generated by metabolic engineering of Corynebacteriumglutamicum characterized on defined glucose/sucrose media as well molasses, side stream from sugar beet processing. While strain C.glutamicum P