Comparison of a prototype magnetoresistive biosensor to standard fluorescent DNA detection

• DOI: 10.1016/j.bios.2003.11.007
• Publication Date: 2003-12-31T11:00:10Z
• Authors: J Schotter; P.B Kamp; A Becker; A Pühler; G Reiss; H Brückl
• Keywords: Biosensing Techniques; DNA; 02 engineering and technology; biosensor; sensors; magnetic markers; Magnetics; Microscopy, Electron; magnetoresistive; Data Interpretation, Statistical; fluorescence; 0210 nano-technology; microarray

We present a comparative analysis of a magnetoresistive biosensor to standard fluorescent DNA detection. The biosensor consists of giant magnetoresistive (GMR) type Cu/Ni(80)Fe(20) multilayers in the second antiferromagnetic coupling maximum. Each of the 206 elements of the magnetoresistive biosensor is patterned into a spiral-shaped line that can cover the area of a typical DNA spot (70 microm diameter). The probe DNA is assembled on top of the sensor elements in different concentrations ranging from 16 pg/microl to 10 ng/microl. Complementary biotin-labeled analyte DNA is hybridized to the probe DNA at a concentration of 10 ng/microl. A number of different commercially available magnetic microspheres are investigated to determine the most appropriate markers. The experimental comparison shows that the relative sensitivity of the magnetoresistive biosensor is superior to the fluorescent detection at low probe DNA concentrations.