Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry

• DOI: 10.1016/j.bios.2016.06.085
• Publication Date: 2016-06-30T05:38:03Z
• Authors: Bo Tian; Zhen Qiu; Jing Ma; Teresa Zardán Góm...; Christer Johansson; Peter Svedlindh; Mattias Strömberg
• Keywords: Zika Virus; Flavivirus; Viremia; Multiplex; Streptavidin

Because of the serological cross-reactivity among flaviviruses, molecular detection methods, such as reverse-transcription polymerase chain reaction (RT-PCR), play an important role in recent Zika outbreak. However, due to limited sensitivity, window RT-PCR for viremia is only about one week after symptom onset. By combining loop-mediated isothermal amplification (LAMP) and AC susceptometry, we demonstrate a rapid homogeneous system virus oligonucleotide. Streptavidin-magnetic nanoparticles (streptavidin-MNPs) are premixed with LAMP reagents including analyte biotinylated primers, their hydrodynamic volumes dramatically increased successful reaction. Analyzed by portable susceptometer, changes volume probed Brownian relaxation frequency shifts, which can be used quantify The proposed recognize 1 aM synthetic oligonucleotide 20% serum total assay time 27min, hopefully widen therefore promising worldwide fever control.