Edge plane pyrolytic graphite as a sensing surface for the determination of fluvoxamine in urine samples of obsessive-compulsive disorder patients

• DOI: 10.1016/j.bios.2020.112489
• Publication Date: 2020-08-13T15:49:01Z
• Authors: Sunita Bishnoi; Ashutosh Sharma; Rahul Singhal; Rajendra N. Goyal
• Keywords: Obsessive-Compulsive Disorder; Fluvoxamine; Humans; Reproducibility of Results; Graphite; Biosensing Techniques; Electrodes; 01 natural sciences; 6. Clean water; 0104 chemical sciences

There is an increasing demand for fast and sensitive determination of antidepressants in human body fluids because of the present scenario of rising depression cases at the global level. A simple and sensitive voltammetric method using edge plane pyrolytic graphite electrode (EPPGE) as a novel sensor is presented for the determination of antidepressant fluvoxamine in urine and blood plasma samples of obsessive-compulsive disorder (OCD) patients. EPPGE is delineated the first time for this determination. EPPGE exhibited strong electrocatalytic activity and enhanced reduction signal towards the sensing of fluvoxamine. Fluvoxamine gave a well-defined reduction peak at ~ - 670 mV using EPPGE. The fluvoxamine reduction peak current was linear to its concentration in the range 5.00 × 10-9 - 0.1 × 10-6 mol L-1 and the limit of detection was found to be 3.5 × 10-9 mol L-1. The pre-eminence of EPPGE over mercury electrodes has been proved in terms of sensitivity and imperative analytical parameters. The pH study reveals the involvement of an equal number of electrons and protons in the reduction reaction mechanism. The frequency study indicated the adsorption controlled irreversible reaction mechanism. The stability and reproducibility of the offered sensor were also found most favorable. The interference study confirmed the optimum selectivity of the proposed sensor. The edge plane pyrolytic graphite sensing platform is recommended as a potential contender for the accurate and fast determination of fluvoxamine in depression medications as well as biological specimens of OCD patients.