Caffeic acid phenethyl ester inhibits the growth of bladder carcinoma cells by upregulating growth differentiation factor 15

• DOI: 10.1016/j.bj.2021.10.006
• Publication Date: 2021-10-19T11:58:35Z
• Authors: Chen-Pang Hou; Ke-Hung Tsui; Kang-Shuo Chang; Hsin-Ching Sung; Shu-Yuan Hsu; Yu-Hsiang Lin; Pei-Shan Yang; Chien-Lun Chen; Tsui-Hsia Feng; Horng-Heng Juang
• Keywords: AMPK; Medicine (General); 0303 health sciences; Growth Differentiation Factor 15; NDRG1; QH301-705.5; Bladder; Urinary Bladder; Carcinoma; Epithelial Cells; CAPE; MAPK; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; GDF15; R5-920; Urinary Bladder Neoplasms; Cell Line, Tumor; Animals; Humans; Original Article; Biology (General)

Caffeic acid phenethyl ester (CAPE), a bioactive component of propolis, has beneficial effects on cancer prevention. Growth differentiation factor 15 (GDF15) is an antitumor gene bladder cancer. Therefore, this study investigated the anti-cancer effect CAPE carcinoma cells and related mechanisms. The expressions GDF15, N-myc downstream-regulated 1 (NDRG1), maspin, activations extracellular signal regulated kinase (ERK), c-jun Nterminal (JNK), p38, 50 adenosine monophosphate-activated protein (AMPK) α1/2 in human after transfection or knockdown were determined by immunoblot, real-time reverse transcriptase-polymerase chain reaction (RT-qPCR), reporter assays. assays 5-ethynyl-2'-deoxyuridine (EdU), CyQUANT cell proliferation, Matrigel invasion, xenograft animal used to assess tumorigenesis. GDF15 expression epithelial was negatively correlated with neoplasia vitro. Also, exhibits fibroblasts smooth muscle cells. CAPE-induced NDRG1 maspin decreased proliferation invasion GDF15-dependent manner suggesting attenuated tumor growth vivo. increased phosphorylation ERK, JNK, AMPKα1/2 modulate expressions. Pretreatments p38 inhibitors partially inhibited inductions NDRG1, maspin. Knockdown Our findings indicate that promising agent for anti-tumor via upregulation GDF15.