Synthesis of mouse centromere-targeted polyamides and physico-chemical studies of their interaction with the target double-stranded DNA

• DOI: 10.1016/j.bmc.2015.06.062
• Publication Date: 2015-07-04T21:31:59Z
• Authors: Karine Nozeret; Marc Bonan; Serguiy M. Yarmoluk; Darya S. Novopashina; Alexandre S. Bout...
• Keywords: Affinities; Binding affinities

Synthetic minor groove-binding pyrrole-imidazole polyamides labeled by fluorophores are promising candidates for fluorescence imaging of double-stranded DNA in isolated chromosomes or fixed and living cells. We synthesized nine hairpin and two head-to-head tandem polyamides targeting repeated sequences from mouse major satellites. Their interaction with synthetic target dsDNA has been studied by physico-chemical methods in vitro before and after coupling to various fluorophores. Great variability in affinities and fluorescence properties reveals a conclusion that these properties do not only rely on recognition rules, but also on other known and unknown structural factors. Individual testing of each probe is needed before cellular applications.