Does early post-operative exercise influence bone healing kinetics? Preclinical evaluation of non-critical sized femur defect healing

• DOI: 10.1016/j.bone.2023.116869
• Publication Date: 2023-08-09T02:42:10Z
• Authors: Omer Suljevic; Uwe Y. Schwarze; Begüm Okutan; Valentin Herber; Helga Lichtenegger; Jan-Eike Freudent...; Annelie-M. Weinberg; Nicole G. Sommer
• Keywords: Ex vivo; Bone remodeling; Bone Formation

Physical exercise is a well-known modality for maintaining healthy locomotor mechanism. A detailed preclinical research on physical effect bone healing kinetics could help to improve the rehabilitation process after fracture treatment and remodeling. Our aim was evaluate of early post-operative microstructural changes in rat model. Twenty Sprague Dawley male rats underwent bi-cortical 1.6 mm hole drilling both femur diaphysis, which (n = 10) continuous treadmill training (TR) over two weeks, while other group assigned non-training (NT) control group. New formation labeling performed by subcutaneous fluorochrome injections at day 5, 14 31. In vivo micro-computed tomography (μCT) scans were once week during 6-week period. Ten animals (five from each group) euthanized 3rd remaining 6th week. Femur samples extracted ex μCT histological evaluation, serum used evaluating alkaline phosphatase (ALP). data demonstrated increased volume surface newly formed defect area TR Bone volume/Tissue (BV/TV) ratio number osteocytes showed an increase 3-week Fluorochrome distances between 5 within Serum ALP level groups 3- 6-weeks. Post-operative increases stimulates new protocol has ended.