Role of endogenous psychosine accumulation in oligodendrocyte differentiation and survival: Implication for Krabbe disease
0301 basic medicine
0303 health sciences
Cell Survival
Blotting, Western
Psychosine
Fluorescent Antibody Technique
Cell Differentiation
Galactosylceramides
Lipid Metabolism
Real-Time Polymerase Chain Reaction
Transfection
Leukodystrophy, Globoid Cell
Rats
Microscopy, Electron
Oligodendroglia
03 medical and health sciences
Microscopy, Fluorescence
In Situ Nick-End Labeling
Animals
Humans
RNA Interference
Myelin Sheath
Galactosylceramidase
DOI:
10.1016/j.brainres.2013.02.024
Publication Date:
2013-02-21T20:16:17Z
AUTHORS (5)
ABSTRACT
Krabbe disease is a lethal, demyelinating condition caused by genetic deficiency of galactocerebrosidase (GALC) and resultant accumulation of its cytotoxic substrate, psychosine (galactosylsphingosine), primarily in oligodendrocytes (OLs). Psychosine is generated by galactosylation of sphingosine by UDP-galactose:ceramide galactosyltransferase (CGT), a galactosylceramide synthesizing enzyme which is primarily expressed in OLs. The expression of CGT and the synthesis of galactosyl-sphingolipids are associated with the terminal differentiation of OL, but little is known about the participation of endogenous psychosine accumulation in OL differentiation under GALC deficient conditions. In this study, we report that accumulation of endogenous psychosine under GALC deficient Krabbe conditions impedes OL differentiation process both by decreasing the expression of myelin lipids and protein and by inducing the cell death of maturating OLs. The psychosine pathology under GALC deficient conditions involves participation of secretory phospholipase A2 (sPLA2) activation and increase in its metabolites, as evidenced by attenuation of psychosine-induced pathology by treatment with pharmacological inhibitor of sPLA2 7,7-dimethyleicosadienoic acid (DEDA). These observations suggest for potential therapeutic efficacy of sPLA2 inhibitor in Krabbe disease.
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CITATIONS (40)
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