Glial cell line-derived neurotrophic factor (GDNF) counteracts hypoxic damage to hippocampal neural network function in vitro

• DOI: 10.1016/j.brainres.2017.10.023
• Publication Date: 2017-11-08T09:01:18Z
• Authors: Tatiana V. Shishkina; Tatiana A. Mishch...; Elena V. Mitroshina; Olesya M. Shirokova; Alexei S. Pimashkin; Innokentiy A. Kas...; Irina V. Mukhina; Victor B. Kazantsev; Maria V. Vedunova
• Keywords: Hypoxia; Viability assay

Glial cell line-derived neurotrophic factor (GDNF) is regarded as a potent neuroprotector and corrector of neural network activity in stress conditions. This work aimed to investigate the effect GDNF on primary hippocampal cultures during acute normobaric hypoxia. Hypoxia induction was performed using day 14 vitro derived from mouse embryos (E18) with preventive addition (1 ng/ml) culture medium 10 min before oxygen deprivation. An analysis spontaneous bioelectrical that included defining internal structure, morphological studies, viability tests post-hypoxic period. study revealed does not influence normoxia but protects death maintains created unique conditions supported cells even cases cellular mitochondrial damage. partially negated consequences hypoxia by influencing synaptic plasticity. Intravital mRNA detection identified fewer GluR2 mRNA-positive cells, whereas preserved number these Activation synthesis subunits AMPA-receptors one possible mechanism neuroprotective action GDNF.