Endothelial progenitor cell transplantation restores vascular injury in mice after whole-brain irradiation

• DOI: 10.1016/j.brainres.2020.147005
• Publication Date: 2020-07-02T15:11:33Z
• Authors: Xurui Huang; Minping Li; Dongxiao Zhou; Zhezhi Deng; Junjie Guo; Haiwei Huang
• Keywords: Brain damage; Endothelial progenitor cell; Pathogenesis

Vascular damage plays an important role in the pathogenesis of radiation-induced brain injury (RBI). Endothelial progenitor cells (EPCs) are responsible for maintaining and repairing endothelial function, have become a promising method treatment cerebrovascular diseases. However, whether EPC transplantation protective RBI has not been fully elucidated. Therefore, present study investigated effects bone marrow-derived whole-brain irradiation (WBI) mouse model. Mice were divided into three groups: control group, group EPCs group. Phosphate buffered saline or intravenously injected mice one week after irradiation, brains analyzed eight weeks injection. Flow cytometry demonstrated that led to significant reduction peripheral blood count; however, increase circulating EPCs. Intravital two-photon imaging western blotting reversed by decreasing blood–brain barrier permeability increasing expression tight junction proteins brain. Additionally, immunofluorescence staining revealed microvascular density was higher than may restore caused WBI barrier, junctions, cerebral capillary density. These results highlight potential beneficial on vascular induced RBI.