Uninterrupted in vivo cerebral microdialysis measures of the acute neurochemical response to a single or repeated concussion in a rat model combining force and rotation

• DOI: 10.1016/j.brainres.2024.148998
• Publication Date: 2024-05-14T06:23:30Z
• Authors: Ian Massé; Luc Moquin; Caroline Bouchard; Alain Gratton; Louis De Beaumont
• Keywords: Microdialysis; Neurochemical

Altered extracellular amino acid concentrations following concussion or mild traumatic brain injury can result in delayed neuronal damage through overactivation of NMDA glutamatergic receptors. However, the consequences repeated concussions prior to complete recovery are not well understood. In this study, we utilized vivo cerebral microdialysis and a weight-drop model investigate acute neurochemical response single adult rats that were fully conscious. A probe was inserted into hippocampus remained place during impact. Primary outcomes included glutamate, GABA, taurine, glycine, glutamine, serine, while secondary righting times excitotoxic indices. Compared sham injury, first resulted significant increases glycine levels, longer times, higher Following second concussion, significantly longer, suggesting cumulative effects only partial observed glutamate taurine levels. GABA indices comparable injury. These findings suggest may induce several acids, could exacerbate neurological symptoms despite less pronounced changes.