Involvement of catalase in the apoptotic mechanism induced by apigenin in HepG2 human hepatoma cells

• DOI: 10.1016/j.cbi.2011.06.009
• Publication Date: 2011-07-06T04:46:54Z
• Authors: Glaucio Valdameri; Marina Trombetta-...; Paulo R. Worfel; Amanda R.A. Pires; Glaucia R. Martinez; Guilhermina R. No...; Silvia M.S.C. Cadena; Mari C. Sogayar; Sheila M.B. Winni...; Maria E.M. Rocha
• Keywords: Viability assay

Apigenin has been reported to inhibit proliferation of cancer cells; however, the mechanism underlying its action is not completely understood. Here, we evaluated effects apigenin on levels expression and activity antioxidant enzymes, involvement ROS in cell death induced by HepG2 human hepatoma cells. Upon treatment with apigenin, cells displayed a reduction viability dose- time-dependent manner, some morphological changes. In addition, generation significantly decreased mRNA catalase intracellular GSH. On other hand, did alter or enzymes. Addition exogenous reduced death. We also demonstrated that are more sensitive apigenin-mediated than primary cultures mouse hepatocytes, suggesting differential toxic effect this agent tumor Our results suggest apigenin-induced apoptosis may be mediated H(2)O(2)-dependent pathway via defenses.