Isothiocyanate l−argininato copper(II) complexes – Solution structure, DNA interaction, anticancer and antimicrobial activity

• DOI: 10.1016/j.cbi.2021.109636
• Publication Date: 2021-09-08T07:35:12Z
• Authors: Agnieszka Wojciec...; Romualda Bregier−...; Urszula K. Komarn...; Sandra Kozieł; Agnieszka Szuster...; Monika Sztandera−...; Anna Jarząb; Zbigniew Staszak; Danuta Witkowska; Agnieszka Bojarsk...; Julia Jezierska
• Keywords: Bioinorganic chemistry

l−argininato copper(II) complexes have been intensively investigated in a variety of diseases due to their therapeutic potential. Here we report the results comprehensive structural studies (ESI−MS, NIR−VIS−UV, EPR) on arising aqueous solutions two ternary with molecular formulas from crystal structures, [Cu(l−Arg)2(NCS)](NCS)·H2O (1) and [Cu(l−Arg)(NCS)2] (2) (l−Arg = l−arginine). Reference systems, Cu(II)/l−Arg/NCS− as well binary Cu(II)/NCS− Cu(II)/l−Arg, were studied parallel by pH−potentiometric titration, EPR VIS spectroscopy characterize stability, structures speciation formed species over broad pH range. Comparative analysis obtained showed that at close 7.0 mononuclear [Cu(l−Arg)2(NCS)]+ is only water solution 1, while equilibrium between [Cu(l−Arg)(SCN)]+ [Cu(l−Arg)2]2+ was detected 2. According DNA binding studies, [Cu(l−Arg)2(NCS)]+, could be considered strong minor groove agents causing, presence H2O2, involvement ROS plasmid damage. The human carcinoma cells (A549 cell line) generally significantly more sensitive cytotoxic antiproliferative effect compounds 1 2 than normal cells. shown antimicrobial activity against bacteria belonging Enterobacteriaceae family.