Single-cell landscapes of primary glioblastomas and matched explants and cell lines show variable retention of inter- and intratumor heterogeneity

• DOI: 10.1016/j.ccell.2022.02.016
• Publication Date: 2022-03-17T16:31:28Z
• Authors: Véronique G. LeBlanc; Diane L. Trinh; Shaghayegh Aslanpour; Martha Hughes; Dorothea Livingstone; Dan Jin; Bo Young Ahn; Michael D. Blough; J. Gregory Cairnc...; Jennifer A. Chan; John J.P. Kelly; Marco A. Marra
• Keywords: Organoid; Exome

Glioblastomas (GBMs) are aggressive brain tumors characterized by extensive inter- and intratumor heterogeneity. Patient-derived models, such as organoids explants, have recently emerged useful models to study heterogeneity, although the extent which they can recapitulate GBM genomic features remains unclear. Here, we analyze bulk exome single-cell genome transcriptome profiles of 12 IDH wild-type GBMs, including two recurrent tumors, patient-derived explants (PDEs) gliomasphere (GS) lines derived from these tumors. We find that PDEs genetically similar to, variably retain gene expression characteristics of, their parent Notably, appear exhibit levels transcriptional heterogeneity compared with whereas GS tend be enriched for cells in a more uniform state. The approaches datasets introduced here will provide valuable resource help guide experiments using GBM-derived especially context studying cellular