Detection and localization of early- and late-stage cancers using platelet RNA

• DOI: 10.1016/j.ccell.2022.08.006
• Publication Date: 2022-09-01T14:51:37Z
• Authors: Sjors G.J.G. In ’...; Mohammad Arkani; Edward Post; Mafalda Antunes-F...; Silvia D’Ambrosi; Daan C.L. Vessies; Lisa Vermunt; Adrienne Vancura; Mirte Muller; Anna-Larissa N. N...; Jihane Tannous; Laura L. Meijer; Tessa Y.S. Le Large; Giulia Mantini; Niels E. Wondergem; Kimberley M. Hein...; Sandra van Wilpe; A. Josien Smits; Esther E.E. Drees; Eva Roos; Cyra E. Leurs; Lee-Ann Tjon Kon Fat; Ewoud J. van der ...; Govert Dwarshuis; Maarten J. Kamphuis; Lisanne E. Visser; Romee Harting; Annemijn Gregory; Markus W. Schweiger; Laurine E. Wedekind; Jip Ramaker; Kenn Zwaan; Heleen Verschueren; Idris Bahce; Adrianus J. de La...; Egbert F. Smit; Michel M. van den...; Koen J. Hartemink; Marijke J.E. Kuij...; Mirjam G.A. oude ...; Arjan W. Griffioen; Rafael Rossel; T. Jeroen N. Hilt...; Elizabeth Lee-Lew...; Kent B. Lewandrowski; Philip C. De Witt...; Mathilde Kouwenhoven; Jaap C. Reijneveld; William P.J. Leen...; Ann Hoeben; Irma M. Verdonck-...; C. René Leemans; Robert J. Baatenb...; Chris H.J. Terhaard; Robert P. Takes; Johannes A. Lange...; Saskia C. de Jager; Adriaan O. Kraaij...; Gerard Pasterkamp; Minke Smits; Jack A. Schalken; Sylwia Łapińska-S...; Anna Łojkowska; Anna J. Żaczek; Henk Lokhorst; Niels W.C.J. van ...; Inger Nijhof; Henk-Jan Prins; Josée M. Zijlstra; Sander Idema; Johannes C. Baayen; Charlotte E. Teun...; Joep Killestein; Marc G. Besselink; Lindsay Brammen; Thomas Bachleitne...; Farrah Mateen; John T.M. Plukker; Michal Heger; Quirijn de Mast; Ton Lisman; D. Michiel Pegtel; Harm-Jan Bogaard; Jacek Jassem; Anna Supernat; Niven Mehra; Winald Gerritsen; Cornelis D. de Kroon; Christianne A.R. Lok; Jurgen M.J. Piek; Neeltje Steeghs; Winan J. van Houdt; Ruud H. Brakenhoff; Gabe S. Sonke; Henk M. Verheul; Elisa Giovannetti; Geert Kazemier; Siamack Sabrkhany; Ed Schuuring; Erik A. Sistermans; Rob Wolthuis; Hanne Meijers-Hei...; Josephine Dorsman; Cees Oudejans; Bauke Ylstra; Bart A. Westerman; Daan van den Broek; Danijela Koppers-...; Pieter Wesseling; R. Jonas A. Nilsson; W. Peter Vandertop; David P. Noske; Bakhos A. Tannous; Nik Sol; Myron G. Best; Thomas Wurdinger
• Keywords: Tumor stage; Cancer stage; Cancer Detection

Cancer patients benefit from early tumor detection since treatment outcomes are more favorable for less advanced cancers. Platelets involved in cancer progression and considered a promising biosource detection, as they alter their RNA content upon local systemic cues. We show that tumor-educated platelet (TEP) RNA-based blood tests enable the of 18 types. With 99% specificity asymptomatic controls, thromboSeq correctly detected presence two-thirds 1,096 samples stage I–IV half 352 I–III tumors. Symptomatic including inflammatory cardiovascular diseases, benign tumors had increased false-positive test results with an average 78%. Moreover, determined site origin five different types over 80% patients. These highlight potential properties TEP-derived panels to supplement current approaches blood-based screening.