Real-time identification of gut microbiota with aminopeptidase N using an activable NIR fluorescent probe

• DOI: 10.1016/j.cclet.2021.03.056
• Publication Date: 2021-03-23T19:42:14Z
• Authors: Lei Feng; Zhenhao Tian; Ming Zhang; Xin He; Xiangge Tian; Zhenlong Yu; Xiaochi Ma; Chao Wang
• Keywords: 01 natural sciences; 0104 chemical sciences

Abstract A NIR fluorescent probe (DDAA) derived from fluorophore DDAO with alanine as the recognition group was developed for sensing aminopeptidase N (APN) in gut microbiota. Using DDAA as the real-time guidance tool for the fluorescence imaging of intestinal microorganism, target bacteria and saccharomycete possessing active APN were identified successfully from human feces.