Silicon-substituted rhodamines for stimulated emission depletion fluorescence nanoscopy

• DOI: 10.1016/j.cclet.2021.06.075
• Publication Date: 2021-07-02T15:55:43Z
• Authors: Ning Wang; Yumeng Hao; Xiaowei Feng; Haidan Zhu; Dazhi Zhang; Ting Wang; Xiaoyan Cui
• Keywords: 0301 basic medicine; 03 medical and health sciences; 01 natural sciences; 0104 chemical sciences

Abstract As an essential part in the toolbox of super-resolution microscopy, stimulated emission depletion (STED) nanoscopy has been widely explored in revealing the substructure and bioactivities in fluorescence imaging. Among the applied STED fluorophores, silicon-substituted rhodamines (SiRs) belong to one of the most extensively employed fluorophores. The carboxy-SiR was favored in STED bioimaging with many advantages, including reliable photostability, cell permeability, tunable fluorogenicity, feasible structural decoration and so on. We reviewed the research of carboxy-SiR in the STED nanoscopy and hopefully this can inspire more efforts in the design and application of STED fluorophores.