The proteomic landscape of synaptic diversity across brain regions and cell types

• DOI: 10.1016/j.cell.2023.09.028
• Publication Date: 2023-11-01T14:30:52Z
• Authors: Marc van Oostrum; Thomas M. Blok; Stefano L. Giando...; Susanne tom Dieck; Georgi Tushev; Nicole Fürst; Julian D. Langer; Erin M. Schuman
• Keywords: Brain Cell; Cell type

Neurons build synaptic contacts using different protein combinations that define the specificity, function, and plasticity potential of synapses; however, diversity proteomes remains largely unexplored. We prepared synaptosomes from 7 transgenic mouse lines with fluorescently labeled presynaptic terminals. Combining microdissection 5 brain regions fluorescent-activated synaptosome sorting (FASS), we isolated analyzed 18 synapse types. discovered ∼1,800 unique synapse-type-enriched proteins allocated thousands to types synapses (https://syndive.org/). identify shared modules highlight proteomic hotspots for specialization. reveal common features striatal dopaminergic proteome discover signatures relate functional properties interneuron classes. This study provides a molecular systems-biology analysis framework integrate information subtypes interest cellular or circuit-level experiments.