Stage-Specific Regulation of Reprogramming to Induced Pluripotent Stem Cells by Wnt Signaling and T Cell Factor Proteins

• DOI: 10.1016/j.celrep.2013.05.015
• Publication Date: 2013-06-20T16:47:58Z
• Authors: Ritchie Ho; Bernadett Papp; Jackson A. Hoffman; Bradley J. Merrill; Kathrin Plath
• Keywords: Reprogramming; Ectopic expression; KLF4; Induced stem cells

Wnt signaling is intrinsic to mouse embryonic stem cell self-renewal. Therefore, it surprising that reprogramming of somatic cells induced pluripotent (iPSCs) not strongly enhanced by signaling. Here, we demonstrate active inhibits the early stage iPSCs, whereas required and even stimulating during late stage. Mechanistically, this biphasic effect accompanied a change in requirement all four its transcriptional effectors: T factor 1 (Tcf1), Lef1, Tcf3, Tcf4. For example, Tcf3 Tcf4 are stimulatory but inhibitory process. Accordingly, ectopic expression combined with loss function enables efficient absence Sox2. Together, our data indicate stepwise process iPSCs critically dependent on stage-specific control action Tcfs