Genome-wide Mapping of Transcriptional Start Sites Defines an Extensive Leaderless Transcriptome in Mycobacterium tuberculosis
Resource
0301 basic medicine
Transcription, Genetic
QH301-705.5
Promoter Regions
03 medical and health sciences
Genetic
Biology (General)
Promoter Regions, Genetic
0303 health sciences
Sequence Analysis, RNA
Gene Expression Profiling
Bacterial
Chromosome Mapping
Gene Expression Regulation, Bacterial
Mycobacterium tuberculosis
3. Good health
Gene Expression Regulation
RNA
Erratum
Transcription Initiation Site
5' Untranslated Regions
Transcriptome
Sequence Analysis
Transcription
DOI:
10.1016/j.celrep.2013.10.031
Publication Date:
2013-11-21T22:07:15Z
AUTHORS (7)
ABSTRACT
Deciphering physiological changes that mediate transition of Mycobacterium tuberculosis between replicating and nonreplicating states is essential to understanding how the pathogen can persist in an individual host for decades. We have combined RNA sequencing (RNA-seq) 5′ triphosphate-enriched libraries with regular RNA-seq characterize architecture expression M. promoters. identified over 4,000 transcriptional start sites (TSSs). Strikingly, 26% genes a primary TSS, site initiation overlapped annotated codon, generating leaderless transcripts lacking UTR and, hence, Shine-Dalgarno sequence commonly used initiate ribosomal engagement eubacteria. Genes encoding proteins active growth functions were markedly depleted from transcriptome, there was significant increase overall representation mRNAs starvation model arrest. The high percentage may particular importance physiology tuberculosis.
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