Genome-wide Mapping of Transcriptional Start Sites Defines an Extensive Leaderless Transcriptome in Mycobacterium tuberculosis

Resource 0301 basic medicine Transcription, Genetic QH301-705.5 Promoter Regions 03 medical and health sciences Genetic Biology (General) Promoter Regions, Genetic 0303 health sciences Sequence Analysis, RNA Gene Expression Profiling Bacterial Chromosome Mapping Gene Expression Regulation, Bacterial Mycobacterium tuberculosis 3. Good health Gene Expression Regulation RNA Erratum Transcription Initiation Site 5' Untranslated Regions Transcriptome Sequence Analysis Transcription
DOI: 10.1016/j.celrep.2013.10.031 Publication Date: 2013-11-21T22:07:15Z
ABSTRACT
Deciphering physiological changes that mediate transition of Mycobacterium tuberculosis between replicating and nonreplicating states is essential to understanding how the pathogen can persist in an individual host for decades. We have combined RNA sequencing (RNA-seq) 5′ triphosphate-enriched libraries with regular RNA-seq characterize architecture expression M. promoters. identified over 4,000 transcriptional start sites (TSSs). Strikingly, 26% genes a primary TSS, site initiation overlapped annotated codon, generating leaderless transcripts lacking UTR and, hence, Shine-Dalgarno sequence commonly used initiate ribosomal engagement eubacteria. Genes encoding proteins active growth functions were markedly depleted from transcriptome, there was significant increase overall representation mRNAs starvation model arrest. The high percentage may particular importance physiology tuberculosis.
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