Fly-FUCCI: A Versatile Tool for Studying Cell Proliferation in Complex Tissues

• DOI: 10.1016/j.celrep.2014.03.020
• Publication Date: 2014-04-13T19:00:17Z
• Authors: Norman Zielke; Jerome Korzelius; Monique van Straaten; Katharina Bender; Gregor F.P. Schuh...; Devanjali Dutta; Jinyi Xiang; Bruce A. Edgar
• Keywords: 0303 health sciences; QH301-705.5; Cell Cycle; Ubiquitination; Cyclin B; Anaphase-Promoting Complex-Cyclosome; Cell Line; 3. Good health; 03 medical and health sciences; Microscopy, Fluorescence; 1300 General Biochemistry, Genetics and Molecular Biology; Organ Specificity; 570 Life sciences; biology; Animals; Drosophila; Biology (General); E2F1 Transcription Factor; 10194 Institute of Neuroinformatics; Cell Proliferation

ISSN:2211-1247<br/>One promising approach for in vivo studies of cell proliferation is the FUCCI system (fluorescent ubiquitination-based cell cycle indicator). Here, we report the development of a Drosophila-specific FUCCI system (Fly-FUCCI) that allows one to distinguish G1, S, and G2 phases of interphase. Fly-FUCCI relies on fluorochrome-tagged degrons from the Cyclin B and E2F1 proteins, which are degraded by the ubiquitin E3-ligases APC/C and CRL4Cdt2, during mitosis or the onset of S phase, respectively. These probes can track cell-cycle patterns in cultured Drosophila cells, eye and wing imaginal discs, salivary glands, the adult midgut, and probably other tissues. To support a broad range of experimental applications, we have generated a toolkit of transgenic Drosophila lines that express the Fly-FUCCI probes under control of the UASt, UASp, QUAS, and ubiquitin promoters. The Fly-FUCCI system should be a valuable tool for visualizing cell-cycle activity during development, tissue homeostasis, and neoplastic growth.<br/>Cell Reports, 7 (2)<br/>ISSN:2666-3864<br/>