CD161 Defines a Transcriptional and Functional Phenotype across Distinct Human T Cell Lineages

• DOI: 10.1016/j.celrep.2014.09.045
• Publication Date: 2014-10-24T06:37:22Z
• Authors: Joannah R. Fergusson; Kira E. Smith; Vicki M. Fleming; Neil Rajoriya; Evan W. Newell; Ruth Simmons; Emanuele Marchi; Sophia Björkander; Yu-Hoi Kang; Leo Swadling; Ayako Kurioka; Natasha Sahgal; Helen Lockstone; Dilair Baban; Gordon J. Freeman; Eva Sverremark-Ek...; Mark M. Davis; Miles P. Davenport; Vanessa Venturi; James E. Ussher; Christian B. Will...; Paul Klenerman
• Keywords: Adult; CD4-Positive T-Lymphocytes; 0301 basic medicine; 570; Transcription, Genetic; QH301-705.5; Receptors, Antigen, T-Cell, alpha-beta; T-Lymphocytes; 610; CD8-Positive T-Lymphocytes; 3101 Biochemistry and Cell Biology; Lymphocyte Activation; Article; 03 medical and health sciences; Genetic; T-Lymphocyte Subsets; Receptors; Genetics; 2.1 Biological and endogenous factors; Humans; Cell Lineage; anzsrc-for: 31 Biological Sciences; Biology (General); alpha-beta; gamma-delta; Principal Component Analysis; anzsrc-for: 3101 Biochemistry and Cell Biology; Interleukin-18; Receptors, Antigen, T-Cell, gamma-delta; T-Cell; Interleukin-12; Phenotype; Antigen; Generic health relevance; Transcription; 31 Biological Sciences; NK Cell Lectin-Like Receptor Subfamily B

The C-type lectin CD161 is expressed by a large proportion of human T lymphocytes all lineages, including population known as mucosal-associated invariant (MAIT) cells. To understand whether different cell subsets expressing have similar properties, we examined these populations in parallel using mass cytometry and mRNA microarray approaches. analysis identified conserved CD161++/MAIT transcriptional signature enriched CD161+CD8+ cells, which can be extended to CD161+ CD4+ CD161+TCRγδ+ Furthermore, this led the identification shared innate-like, TCR-independent response interleukin (IL)-12 plus IL-18 CD161-expressing populations. This was independent regulation CD161, acted costimulatory molecule context receptor stimulation. Expression hence identifies functional phenotype, across both (TCR) expression lineage.