Disease Modeling and Phenotypic Drug Screening for Diabetic Cardiomyopathy using Human Induced Pluripotent Stem Cells

Diabetic Cardiomyopathy Clinical phenotype Phenotypic screening
DOI: 10.1016/j.celrep.2014.09.055 Publication Date: 2014-10-30T17:01:32Z
ABSTRACT
Diabetic cardiomyopathy is a complication of type 2 diabetes, with known contributions lifestyle and genetics. We develop environmentally genetically driven in vitro models the condition using human-induced-pluripotent-stem-cell-derived cardiomyocytes. First, we mimic diabetic clinical chemistry to induce phenotypic surrogate cardiomyopathy, observing structural functional disarray. Next, consider genetic effects by deriving cardiomyocytes from two patients variable disease progression. The cardiomyopathic phenotype recapitulated patient-specific cells basally, severity dependent on their original status. These are incorporated into successive levels screening platform, identifying drugs that preserve cardiomyocyte during stress. In this work, present induced pluripotent stem cell (iPSC) model complex metabolic condition, showing power technique for discovery testing therapeutic strategies ever-increasing significance.
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