Cellular Taxonomy of the Mouse Striatum as Revealed by Single-Cell RNA-Seq

• DOI: 10.1016/j.celrep.2016.06.059
• Publication Date: 2016-07-16T01:14:57Z
• Authors: Ozgun Gokce; Geoffrey M. Stanley; Barbara Treutlein; Norma F. Neff; J. Gray Camp; Robert C. Malenka; Patrick E. Rothwell; Marc V. Fuccillo; Thomas C. Südhof; Stephen R. Quake
• Keywords: Cell type; Single-Cell Analysis; RNA-Seq; Medium spiny neuron

The striatum contributes to many cognitive processes and disorders, but its cell types are incompletely characterized. We show that microfluidic FACS-based single-cell RNA sequencing of mouse provides a well-resolved classification striatal type diversity. Transcriptome analysis revealed ten differentiated, distinct types, including neurons, astrocytes, oligodendrocytes, ependymal, immune, vascular cells, enabled the discovery numerous marker genes. Furthermore, we identified two discrete subtypes medium spiny neurons (MSNs) have specific markers overexpress genes linked disorders addiction. also describe continuous cellular identities, which increase heterogeneity within types. Finally, type-specific transcription splicing factors shape identities by regulating expression patterns. Our findings suggest functional diversity complex tissue arises from small number can exist in spectrum states.