Differential Chromosomal Localization of Centromeric Histone CENP-A Contributes to Nematode Programmed DNA Elimination

• DOI: 10.1016/j.celrep.2016.07.079
• Publication Date: 2016-08-22T20:52:18Z
• Authors: Yuanyuan Kang; Jianbin Wang; Ashley Neff; Stella Kratzer; Hiroshi Kimura; Richard E. Davis
• Keywords: 570; Embryo, Nonmammalian; QH301-705.5; Chromosomal Proteins, Non-Histone; NDC80; Centromere; chromosome segregation; Gene Expression; Mitosis; Autoantigens; Microtubules; 630; programmed DNA elimination; 03 medical and health sciences; Chromosome Segregation; Animals; Biology (General); Kinetochores; Ascaris suum; Genome, Helminth; 0303 health sciences; Ascaris; Chromosome Mapping; holocentromere; Helminth Proteins; Chromatin; kinetochore; centromere; CENP-A; Centromere Protein A; CENP-C

The stability of the genome is paramount to organisms. However, diverse eukaryotes carry out programmed DNA elimination in which portions or entire chromsomes are lost early development during sex determination. During parasitic nematode, Ascaris suum, 13% eliminated. How different genomic segments reproducibly retained discarded unknown. Here, we show that centromeric histone CENP-A localization plays a key role this process. We chromosomes holocentric germline mitoses, with distributed along their length. Prior four-cell embryo, significantly diminished chromosome regions will be lost. This leads absence kinetochores and microtubule attachment sites necessary for segregation, resulting loss these upon mitosis. Our data suggest changes specify elimination.