The RNA-Binding Protein SYNCRIP Is a Component of the Hepatocyte Exosomal Machinery Controlling MicroRNA Sorting

• DOI: 10.1016/j.celrep.2016.09.031
• Publication Date: 2016-10-11T16:00:40Z
• Authors: Laura Santangelo; Giorgio Giurato; Carla Cicchini; Claudia Montaldo; Carmine Mancone; Roberta Tarallo; Cecilia Battistelli; Tonino Alonzi; Alessandro Weisz; Marco Tripodi
• Keywords: Exosome

Despite clear evidence that exosomal microRNAs (miRNAs) are able to modulate the cellular microenvironment and RNA cargo selection is deregulated in pathological conditions, mechanisms controlling specific sorting into extracellular vesicles still poorly understood. Here, we identified binding protein SYNCRIP (synaptotagmin-binding cytoplasmic RNA-interacting protein; also known as hnRNP-Q or NSAP1) a component of hepatocyte miRNA machinery. knockdown impairs miRNAs exosomes. Furthermore, directly binds enriched exosomes sharing common extra-seed sequence (hEXO motif). The hEXO motif has role regulation localization, since embedment this exported enhances its loading This provides insights process. Moreover, these findings open way for possible selective modification cargo.