Diverse Central Projection Patterns of Retinal Ganglion Cells

• DOI: 10.1016/j.celrep.2017.01.075
• Publication Date: 2017-02-22T10:16:04Z
• Authors: Emily M. Martersteck; Karla E. Hirokawa; Mariah Evarts; Amy Bernard; Xin Duan; Yang Li; Lydia Ng; Seung W. Oh; Benjamin Ouellette; Joshua J. Royall; Michelle Stoecklin; Quanxin Wang; Hongkui Zeng; Joshua R. Sanes; Julie A. Harris
• Keywords: Retinal Ganglion Cells; Superior Colliculi; 0303 health sciences; Integrases; QH301-705.5; axon projections; Mice, Transgenic; retinal ganglion cell types; connectivity mapping; Retina; Mice; 03 medical and health sciences; Cre driver; Animals; Visual Pathways; Biology (General)

Highlights•Anatomical characterization of Cre expression in the retina 88 driver lines•Morphological and histochemical classification Cre+ RGC types 26 lines•High resolution whole brain imaging labeled retinal axons reveals central targets•Correspondences described between line projection patternsSummaryUnderstanding how >30 ganglion cells (RGCs) mouse each contribute to visual processing will require more tools that label manipulate specific RGCs. We screened analyzed recombinase using transgenic lines. In many lines, was expressed multiple cell classes, but several exhibited selective expression. comprehensively mapped projections from RGCs lines viral tracers, high-throughput imaging, a data pipeline. identified over 50 retinorecipient regions present quantitative retina-to-brain connectivity map, enabling comparisons target-specificity across Projections two major targets were notably correlated: projecting outer shell or core lateral geniculate projected superficial deep layers within superior colliculus, respectively. Retinal images are available online at http://connectivity.brain-map.org.Graphical abstract