DNA Methylome Analysis Identifies Transcription Factor-Based Epigenomic Signatures of Multilineage Competence in Neural Stem/Progenitor Cells

• DOI: 10.1016/j.celrep.2017.08.086
• Publication Date: 2017-09-19T23:16:36Z
• Authors: Tsukasa Sanosaka; Takuya Imamura; Nobuhiko Hamazaki; MuhChyi Chai; Katsuhide Igarashi; Maky Ideta-Otsuka; Fumihito Miura; Takashi Ito; Nobuyuki Fujii; Kazuho Ikeo; Kinichi Nakashima
• Keywords: Epigenomics; DNA demethylation; Epigenome

Regulation of the epigenome during in vivo specification brain stem cells is still poorly understood. Here, we report DNA methylome analyses directly sampled cortical neural and progenitor (NS/PCs) at different development stages, as well those terminally differentiated neurons, astrocytes, oligodendrocytes. We found that sequential NS/PCs regulated by two successive waves demethylation early late which are responsible for establishment neuron- glia-specific low-methylated regions (LMRs), respectively. The regulatory role gliogenic genes was substantiated enrichment nuclear factor I (NFI)-binding sites. provide evidence de novo methylation neuron-specific LMRs establishes epigenotypes, essentially silencing neuronal genes. Our data highlight implications dynamics shaping epigenomic features confer differentiation potential sequentially development.