Nap1l1 Controls Embryonic Neural Progenitor Cell Proliferation and Differentiation in the Developing Brain

• DOI: 10.1016/j.celrep.2018.02.019
• Publication Date: 2018-02-27T16:37:57Z
• Authors: Huimin Qiao; Yanxin Li; Chao Feng; Shuguang Duo; Fen Ji; Jianwei Jiao
• Keywords: Progenitor; Brain Development; Neural cell

Highlights•Nap1l1 knockdown or knockout causes abnormal embryonic neurogenesis•Nap1l1 depletion induces neuronal dendrites and morphology•Nap1l1 regulates cortical development via regulation of RassF10SummaryThe precise function role nucleosome assembly protein 1-like 1 (Nap1l1) in brain are unclear. Here, we find that Nap1l1 decreases neural progenitor cell (NPC) proliferation premature differentiation during development. A similar deficiency neurogenesis was observed (KO) mice, which were generated using the CRISPR-Cas9 system. RNA sequencing (RNA-seq) analysis indicates Ras-associated domain family member 10 (RassF10) may be downstream target Nap1l1. Furthermore, found RassF10 expression by promoting SETD1A-mediated H3K4 trimethylation at promoter. KO defects rescued overexpression, suggesting controls NPC through RassF10. Our findings reveal an essential for histone chaperone early development.Graphical abstract