Qualitative Profiling of the Humoral Immune Response Elicited by rVSV-ΔG-EBOV-GP Using a Systems Serology Assay, Domain Programmable Arrays

570 0303 health sciences Terrorism Studies Other Public Affairs 610 Defense and Security Studies Ebolavirus Immunity, Humoral 3. Good health Macaca fascicularis Mice 03 medical and health sciences Public Policy and Public Administration Public Affairs Animals Humans Science and Technology Policy Ebola Vaccines Emergency and Disaster Management
DOI: 10.1016/j.celrep.2018.06.077 Publication Date: 2018-07-24T17:11:10Z
ABSTRACT
Development of an effective vaccine became a worldwide priority after the devastating 2013-2016 Ebola disease outbreak. To qualitatively profile the humoral response against advanced filovirus vaccine candidates, we developed Domain Programmable Arrays (DPA), a systems serology platform to identify epitopes targeted after vaccination or filovirus infection. We optimized the assay using a panel of well-characterized monoclonal antibodies. After optimization, we utilized the system to longitudinally characterize the immunoglobulin (Ig) isotype-specific responses in non-human primates vaccinated with rVSV-ΔG-EBOV-glycoprotein (GP). Strikingly, we observed that, although the IgM response was directed against epitopes over the whole GP, the IgG and IgA responses were almost exclusively directed against the mucin-like domain (MLD) of the glycan cap. Further research will be needed to characterize this possible biased IgG and IgA response toward the MLD, but the results corroborate that DPA is a valuable tool to qualitatively measure the humoral response after vaccination.
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