Qualitative Profiling of the Humoral Immune Response Elicited by rVSV-ΔG-EBOV-GP Using a Systems Serology Assay, Domain Programmable Arrays

• DOI: 10.1016/j.celrep.2018.06.077
• Publication Date: 2018-07-24T17:11:10Z
• Authors: Mariano Sanchez-L...; Daniel S. Reyes; Jeanette C. Gonzalez; Karla Y. Garcia; Erika C. Villa; Bradley P. Pfeffer; John C. Trefry; Jeffrey R. Kugelman; Margaret L. Pitt; Gustavo F. Palacios
• Keywords: Profiling (computer programming); Vero cell

Development of an effective vaccine became a worldwide priority after the devastating 2013–2016 Ebola disease outbreak. To qualitatively profile humoral response against advanced filovirus candidates, we developed Domain Programmable Arrays (DPA), systems serology platform to identify epitopes targeted vaccination or infection. We optimized assay using panel well-characterized monoclonal antibodies. After optimization, utilized system longitudinally characterize immunoglobulin (Ig) isotype-specific responses in non-human primates vaccinated with rVSV-ΔG-EBOV-glycoprotein (GP). Strikingly, observed that, although IgM was directed over whole GP, IgG and IgA were almost exclusively mucin-like domain (MLD) glycan cap. Further research will be needed this possible biased toward MLD, but results corroborate that DPA is valuable tool measure vaccination.