Chromatin Modifiers SET-25 and SET-32 Are Required for Establishment but Not Long-Term Maintenance of Transgenerational Epigenetic Inheritance
Male
0301 basic medicine
QH301-705.5
Longevity
Inheritance Patterns
Methylation
set-25
not
maintenance
Epigenesis, Genetic
Histones
03 medical and health sciences
establishment
inheritance
Animals
transgenerational
Gene Silencing
Biology (General)
RNA, Small Interfering
Caenorhabditis elegans
Caenorhabditis elegans Proteins
long-term
Models, Genetic
Lysine
required
set-32
Histone-Lysine N-Methyltransferase
Chromatin
modifiers
Germ Cells
Phenotype
Mutation
Histone Methyltransferases
chromatin
RNA
but
epigenetic
DOI:
10.1016/j.celrep.2018.10.085
Publication Date:
2018-11-20T15:41:46Z
AUTHORS (8)
ABSTRACT
Some epigenetic modifications are inherited from one generation to the next, providing a potential mechanism for the inheritance of environmentally acquired traits. Transgenerational inheritance of RNAi phenotypes in Caenorhabditis elegans provides an excellent model to study this phenomenon, and although studies have implicated both chromatin modifications and small RNA pathways in heritable silencing, their relative contributions remain unclear. Here, we demonstrate that the putative histone methyltransferases SET-25 and SET-32 are required for establishment of a transgenerational silencing signal but not for long-term maintenance of this signal between subsequent generations, suggesting that transgenerational epigenetic inheritance is a multi-step process with distinct genetic requirements for establishment and maintenance of heritable silencing. Furthermore, small RNA sequencing reveals that the abundance of secondary siRNAs (thought to be the effector molecules of heritable silencing) does not correlate with silencing phenotypes. Together, our results suggest that the current mechanistic models of epigenetic inheritance are incomplete.
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CITATIONS (56)
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