Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair

• DOI: 10.1016/j.celrep.2019.02.016
• Publication Date: 2019-03-05T11:08:50Z
• Authors: Thomas Colunga; Miranda Hayworth; Sebastian Kreß; David M. Reynolds; Luoman Chen; Kristopher L. Nazor; Johannes Baur; Amar M. Singh; Jeanne F. Loring; Marco Metzger; Stephen Dalton
• Keywords: Decellularization; Mesothelium; Multipotent Stem Cell

In this report we describe a human pluripotent stem cell-derived vascular progenitor (MesoT) cell of the mesothelium lineage. MesoT cells are multipotent and generate smooth muscle cells, endothelial pericytes self-assemble into vessel-like networks in vitro. transplanted mechanically damaged neonatal mouse heart migrate injured tissue contribute to nascent coronary vessels repair zone. When seeded onto decellularized scaffolds, differentiate major lineages vasculature capable supporting peripheral blood flow following transplantation. These findings demonstrate vivo functionality potential utility engineering applications.